human proteins β actin c4 Search Results


human proteins β actin c4  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology human proteins β actin c4
EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.
Human Proteins β Actin C4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alix  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology alix
EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The <t>tetraspanin</t> <t>proteins</t> CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein <t>Alix</t> were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.
Alix, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proteins+%CE%B2+actin+c4/Alix/pmc05451862-69-20-26
Average 96 stars, based on 1 article reviews
alix - by Bioz Stars, 2026-11
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cd59  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology cd59
Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), <t>CD59,</t> β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.
Cd59, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proteins+%CE%B2+actin+c4/H-7/pmc05451862-69-22-26
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β catenin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology β catenin
Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, <t>β</t> <t>-catenin,</t> and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.
β Catenin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proteins+%CE%B2+actin+c4/%CE%B2-catenin+Antibody/pmc05451862-69-13-26
Average 96 stars, based on 1 article reviews
β catenin - by Bioz Stars, 2026-11
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Image Search Results


EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

Journal: Disease Markers

Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

doi: 10.1155/2017/9653194

Figure Lengend Snippet: EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

Techniques: Isolation, Western Blot, Negative Control, Control, Molecular Weight

Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Journal: Disease Markers

Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

doi: 10.1155/2017/9653194

Figure Lengend Snippet: Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

Techniques: Isolation, Western Blot, Negative Control, Control, Zymography, Molecular Weight

EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

Journal: Disease Markers

Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

doi: 10.1155/2017/9653194

Figure Lengend Snippet: EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

Techniques: Isolation, Western Blot, Negative Control, Control, Molecular Weight

Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Journal: Disease Markers

Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

doi: 10.1155/2017/9653194

Figure Lengend Snippet: Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

Techniques: Isolation, Western Blot, Negative Control, Control, Zymography, Molecular Weight

Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Journal: Disease Markers

Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

doi: 10.1155/2017/9653194

Figure Lengend Snippet: Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

Techniques: Isolation, Western Blot, Negative Control, Control, Zymography, Molecular Weight